WebJul 22, 2016 · Background Sodium butyrate (SB) is reported to regulate lipid metabolism in mammals, and the relationship between maternal nutrition and offspring growth has drawn much attention in the last several years. Methods To elucidate the effects of maternal dietary SB supplementation on hepatic lipid metabolism in weaning rats, we fed 16 primiparous … WebJan 26, 2024 · Pyroptosis is a type of programmed cell death triggered by a variety of exogenous stimuli, playing important roles in the development of cells. Recent studies have shown that pyroptosis also occurs...
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WebMay 31, 2024 · Gut microbiota plays important roles in health maintenance and diseases. Physical exercise has been demonstrated to be able to modulate gut microbiota. However, the potential role of gut microbiome in exercise protection to myocardial infarction (MI) remains unclear. Here, we discovered exercise training ameliorated cardiac dysfunction … Webgoat anti-mouse IgG-HRP is a high quality horseradish peroxidase conjugated secondary antibody recommended for use in WB. Starting dilution: 1:2000 how to subtract manually
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WebMAB318, 1:2000) or β-actin (Bioworld, AP0060, 1:5000). After washing with Tris-buffered saline containing 0.1% Tween-20, the membranes were incubated with HRP-conjugated secondary antibodies. WebSep 17, 2024 · AP0060, Bioworld, 1:5000). Then the membrane was washed by TBST (0.1% Tween 20) and incubated with secondary antibody (Cat.RM3002, Beijing Ray Antibody Biotech, 1:10000). The ECL detection reagent was used to visualize the protein bands in the membrane. The quantity of the bands was analyzed with ImageJ. WebMay 7, 2024 · To validate whether these muscle specific miRNAs are m6A modified, we immunoprecipitated m6A modified RNAs from differentiated C2C12 cells and then performed real-time RT-PCR to detect the pri-miRNA levels (Fig. 1 B).Comparing to IgG immunoprecipitated RNAs, miR-21, a known m6A-modified miRNA, was significantly … how to subtract lunch time in excel