Biotin ctp
WebJul 21, 2016 · 2-Biotin run-on 2× reaction mix. i. Transfer a 28-μl aliquot of NRO master mix to a microcentrifuge tube. ii. Add 5 μl each of biotin-11-CTP (1 mM) and biotin-11-UTP (1 mM) to the NRO master ... WebOptimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11 …
Biotin ctp
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WebBiotin-14-CTP is a CTP analog that contains biotin attached at the N4-position via a 14-atom linker. It is provided as a 10 mM solution in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. The amount of material provided is sufficient for up to 20 transcription reactions (1 µg template) using T7 RNA WebBiotin-16-dCTP is a modified deoxynucleoside triphosphate (dNTP), serving as a convenient tool for the enzymatic introduction of the biotin moiety into a nucleic acid target of interest. The biotin moiety exhibits a very strong affinity for streptavidin. The strength and specificity of this interaction has been exploited in a variety of ...
WebBiotin 11-ddCTP C28H44N7O15P3S CID 10306315 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological ... WebBiotin-11-CTP, >95%. Life Sciences & Diagnostics A Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance …
WebBiotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; incorporation into DNA proceeds as with natural dCTP.Biotin-11-dCTP is a substrate for Klenow fragment, T4 and Taq DNA-polymerases, reverse transcriptase, and terminal transferase. WebDec 16, 2024 · In our design, the biotin CTP could be changed to any other biotin nucleobase depending on which nucleobase presents the most in the probe sequence to achieve optimal labeling efficiency; iii) the ratio between the forward primer and the reverse primer in the biotin-probe synthesis process was adjusted to 1:4 to allow the formation …
WebA fairly wide range of concentrations of Biotin-UTP (25–60%) can be used for acceptable detection, whereas at lower concentrations the signal drops dramatically, and at higher …
WebBiotin-14-CTP is a CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker. Biotin-14-CTP can be efficiently incorporated into RNA … fluke 67 max thermometerWebShelf Life: 12 months after date of delivery. Molecular Formula: C 28 H 44 N 7 O 17 P 3 S (free acid) Molecular Weight: 875.67 g/mol (free acid) Exact Mass: 875.17 g/mol (free … green famous charactersWebBiotin-14-dCTP is a dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, … green fancy short dresseshttp://www.delta-f.com/details/1159044 fluke 714 thermometerWebAfter incorporating a pulse of a labelled nucleotide , such as 5-bromouridine 5′-triphosphate (BrUTP) or biotin-CTP, into permeabilized mammalian cells, Cook and colleaguesnoted … greenfan fireplace fansgreen fancy dressWeb이 문서는 영어 위키백과의 Biotin 문서를 번역하여 문서의 내용을 확장할 필요가 있습니다. 중요한 번역 안내를 보려면 [펼치기]를 클릭하십시오. 신뢰성 있고 확인할 수 있는 출처가 제시되도록 번역하여 주십시오. green fantasy dvd collection #98